如果小伙伴們急著發第一篇SCI文章，著急畢業，你實在應該考慮下分析生物化學。最近，小編又發現了一個“短平快”的文章，題為“Optimization of blocking conditions for fluorescent Western blot”，更令人瞠目結舌的是，這篇文章只有一個作者，只是摸索了一個條件，發了一篇IF 2.4（Analytical biochemistry）的文章。下面小編分解下這篇文章，主要從寫法上面分析。先看標題。這篇文章的標題是標準的分析化學領域標題，沒有（也不需要）故事性：在XXX方面優化XXXX條件。再看摘要，
急速時時彩
標準的倒金字塔結構，四段式寫法。第一句 “locking conditions are critical for reducing background and non-specific signals in Western blot (WB)”錛 框定大範圍（Western blot）；第二句“For fluorescent WB錛 however錛 blockers may bring in additional autofluorescence錛 and optimal blocking conditions have been less well established”，縮小範圍 （fluorescent WB），並指出問題 “XXXX have been less well established”。緊接著介紹“results”。最後幹脆利落地總結下，“This work provides a reference for cost-effective blocking conditions for fluorescent WB。”三看前言。作者直入正題，先稱贊fluorescent WB 很常用，很好，及其分類。然後迅速轉折，“fluorescent WB often suffers xxxxx”。緊接著，作者指出解決辦法，“ Blocking conditions are critical for XXXXXX ”，提到了目前的現狀，“Currently錛 there are mainly two types of imagers”。再次，作者簡明扼要地介紹了“results”，用的方式是“In this work錛 XXXX were systematically investigated，XXXXXX錛 revealing that XXXXX”。最後，
花小豬打車遭多地叫停
總結“High auto fluorescent background can be avoided by choosing a proper blocker for a given detection channel”.四看材料與方法。常規寫法，“不求出彩，但求詳細”，多採用一般過去時被動式。例子如下：五看“Results” and “Discussion”。“Results”寫得非常詳細，從3.1寫到了3.17，
丁俊暉不敵奧沙利文
很少遇到這樣的作者和文章。“Discussion”也總結得很細致。由于小編尚未思索清楚如何和大家討論，暫時不詳談。最後看“Conclusions”。作者先總結了下問題，“The background in a fluorescent WB is much more complex than in a chemiluminescent WB錛 including both the autofluorescent background and the background caused by non-specific binding of the antibodies to the membrane.” 然後再說自己優化過的參數。最後，不溫不火的來了句，實在不行就換個常規方法吧。分析生物化學是一門由分析化學和生物化學組成的交叉學科，其目的就是通過各種條件優化來提高檢測方法的特異性和敏感性。這篇文章主要是提高檢測的敏感性（通過降低噪音的方式）。該文沒有看出明顯地設置對照。此外，這篇文章只有一個作者。小編查了下，是來自德國Leibniz Graduate school的中國籍研究人員 Yan Cui。Yan Cui應該是一位博後，主要研究方向是神經科學。為了解決自己的試驗問題，TA特意優化了熒光WB的試驗條件，順便發表了篇SCI文章，
Yan Cui自己設計試驗，自己動手試驗，自己寫作，獨立發表，並且是唯一作者。 如果您覺得以上內容對您有幫助，請幫我們推薦並分享，您的支持是我們最大的動力！ijournal：高顏值的期刊檢索網站，助您快速找到理想目標期刊（weixin小程序也上線了哦）投必得：全專業中英文論文潤色編輯助力您的論文快速發表，點擊了解業務詳情Local auxin biosynthesis is required for root regeneration after wounding第一作者：Rotem Matosevich第一單位：耶路撒冷希伯來大學通訊作者：Idan Efroni Abstract 背景回顧: The root meristem can regenerate following removal of its stem-cell niche by recruitment of remnant cells from the stump.提出問題：Regeneration is initiated by rapid accumulation of auxin near the injury site but the source of this auxin is unknown.主要發現：Here錛 we show that auxin accumulation arises from the activity of multiple auxin biosynthetic sources that are newly specified near the cut site and that their continuous activity is required for the regeneration process.結果-Auxin_PIN：Auxin synthesis is highly localized while PIN-mediated transport is dispensable for auxin accumulation and tip regeneration.結果-ERF115：Roots lacking the activity of the regeneration competence factor ERF115錛 or that are dissected at a zone of low regeneration potential錛 fail to activate local auxin sources. Remarkably錛 restoring auxin supply is sufficient to confer regeneration capacity to these recalcitrant tissues.結論：We suggest that regeneration competence relies on the ability to specify new local auxin sources in a precise temporal pattern. 摘要 植物根的分生組織能夠在移除掉本身幹細胞龕之後通過招募殘留的細胞來再生。再生起始于受傷部位附近的快速生長素積累，但是生長素來源于哪並不清楚。本文中，作者發現生長素積累來自于多個生長素合成源的激活，這些生長素合成源均是在受傷部位附近新形成的，並且其持續的活性對于整個再生過程是必需的。生長素的合成是高度聚集的，
